Laser capture microdissection under a microscope using a software interface a tissue section typically 5 50 micrometres thick is viewed and individual cells or clusters of cells are identified either manually or in semi automated or more fully automated ways allowing the imaging and then automatic selection of targets for isolation. 1 department of biomedical research university of veterinary medicine vienna. When applied to plant cells and especially to woody tissues lcm requires extensive optimization to overcome such factors as rigid cell walls large central vacuoles intercellular spaces and technical issues with thickness and flatness of the sections.
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